Lab Protocols
In keeping with the open science practices, all experimental protocols and procedures used in our lab are published as open-access resources and are freely available for other to use. For inquiries regarding specific methodologies or protocols not found here, please contact our research team via the Contact page found in MENU button at the top right of the page.
Agarose Gel Electrophoresis
Gel electrophoresis allows for the isolation and purification of DNA fragments based on size.
Bacterial Colony PCR
Colony PCR allows for a quick and easy verification of successful transformation.
Heat Shock Transformation of Bacteria
Heat shock harnesses the power of rapid temperature change to create temporary openings in the bacterial cell membrane useful for incorporation of plasmids or other genomic material.
Gibson Overlap PCR
Gibson overlap PCR kits like NEBuilder allow for the seamless cloning of DNA fragments into plasmids with the use of 20 bp complimentary sequence instead of relying on overhangs produced by restriction enzymes.
